The E3101 Optical Tweezer System
The E3101 Optical Tweezer System is an augmented version of our E3100, offering a simple, pre-aligned and fully interlocked, laser workstation for single beam trapping and manipulation of micron sized particles. It incorporates all the key items of hardware within a stylish self contained desktop instrument.
The standard version has been designed as an economical entry-level product to enable non-specialist users to evaluate the technique in a safe class 1 laser environment. For more advanced applications, a quadrant photo detector (QPD) system can be incorporated to offer force measurement.
Output from the internal colour CCD camera can be displayed either directly on a monitor via the S-video connection or on a computer fitted with a framegrabber.
Features
- Cell manipulation via conjugate beam steering optics or precision XY stage
- XYZ trapping using variable focus stage
- All essential hardware is within the desktop workstation
- Laser in fully interlocked Class 1 enclosure
- Rotation of birefringent particles with optional polarization optics
Cell manipulation is carried out by using either the conjugate beam steering optics or the precision XY stage. Trapping in X,Y and Z axes is also possible using the variable focus stage, where particles can be lifted above the rest of the sample. For applications involving rotation of birefringent particles an optional polarization optic and rotation mount can be added.
Samples on a microscope slide are inserted into the sample holder (see right) which is mounted on an XYZ stage. Access to the sample holder is via an interlocked door. The diode laser is mounted within the enclosure and in the standard system comprises either a 685nm (40 mW) or 785nm (56 mW) source. Each laser features a circular near Gaussian profile with diffraction-limited performance. The x100 (NA 1.25) oil immersion objective in conjunction with the high beam quality of the laser provides a very small focused spot allowing the trapping of individual particles.
Specifications
Model E3101 Optical Tweezer System comprises:
- Fully interlocked laser enclosure
- Internal diode laser source (choice of either 685nm or 785nm), with adjustable output power
- 100x oil immersion objective with 1.25 NA for laser focusing and effective particle trapping
- Dichroic Visible/IR mirror
- Conjugate beam steering optics
- Sample stage on XYZ micropositioner with manual controls
- Backlight illumination with adjustable intensity cool light source
- Colour CCD camera with video out
- Laser blocking filter
- Variable focus stage for XYZ trapping
- Laser power monitor and display
- Optional QPD Force Measurement attachment
The E3101 is manufactured under license from The University of St. Andrews.
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