technologiclee: Jun 8, 2010

---------- Forwarded message ----------
From: "Anselm Levskaya" <levskaya@gmail.com>
Date: Jun 8, 2010 4:45 PM
Subject: Re: Trying to build GPCR reporter system
To: <diybio@googlegroups.com>

Nathan,

I'm not sure how far you've read along for this project yet, but are
you aware of the GPCR-Gpa1 fusion strategy for splicing ~arbitrary
metazoans GPCRs into the yeast pheromone sensing pathway? This allows
one to assay receptor activity using a yeast pheromone reporter strain
(lacZ or GFP). Many such chimeric strains have been produced to date.

Here are a few open links:
http://www.slideshare.net/beneshjoseph/functional-analysis-of-heterologous-gpcr-signaling-pathways-in-yeast
http://anselmlevskaya.com/papers/yeastGPCR.pdf
http://peds.oxfordjournals.org/cgi/content/full/19/1/1
http://anselmlevskaya.com/papers/yeastGPCR2.pdf

-a

On Thu, Jun 3, 2010 at 12:30, Nathan McCorkle <nmz787@gmail.com> wrote:
> Hello,
>
> Recently I hav...

---------- Forwarded message ----------
From: "Bryan Bishop" <kanzure@gmail.com>
Date: Jun 8, 2010 9:00 AM
Subject: Re: Is there a tool or database for ligand binding domains?
To: <diybio@googlegroups.com>, <kanzure@gmail.com>

On Mon, Jun 7, 2010 at 3:30 PM, Nathan McCorkle wrote:
> I'm working with a G protein-coupled recept...
LMGTFY.

http://bindingdb.org/
http://www.bindingmoad.org/
http://sw16.im.med.umich.edu/databases/pdbbind/index.jsp
http://pdbbind.org/
http://pdbbind.org.cn/

Usually there's some motif that serves as the ligand binding domain.

- Bryan
http://heybryan.org/
1 512 203 0507

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