Fwd: Sequencer update

---------- Forwarded message ----------
From: TS Nowlan <apokruphos@gmail.com>
Date: Thu, Apr 15, 2010 at 1:20 PM
Subject: Re: Sequencer update
To: diybio@googlegroups.com


We have an ABI PRISM 310, an older model single-capillary sequencer. There is an electrical fault somewhere, which we are still in the process of hunting down / fixing. I'm not personally familiar with its materials (I'm used to just working with the data afterwards), but once we have it up and running I'm sure we'd happy to help test out your kit however we can.

-Tor

On Thu, Apr 15, 2010 at 11:12 AM, Stanley Han <zhan528@gmail.com> wrote:

What type of ABI instrument do you have? I wonder if you will be interested in testing my dye removal kit with your instrument if you use capillery sequencer---PureSEQ?

On Thu, Apr 15, 2010 at 2:10 PM, Stanley Han <zhan528@gmail.com> wrote:

You need a enzyme that incorporate ddNTP efficiently for sequencing not regular Taq. Bigdye use this kind of Taq.

On Wed, Apr 14, 2010 at 7:39 PM, Mackenzie Cowell <mac@diybio.org> wrote:

I'm organizing a PCR genotyping workshop next weekend and have been looking for a diybio-friendly sanger sequencing provider (haven't actually made accounts or taked to anyone at any companies or sequencing centers yet, though).  It would be extra cool to do some of the sequencing with diybio!  If I got reagents, could you guys do some sequencing on one or two samples?

Also, a general question about sanger sequencing: I'm probably going to use Taq, but I might purchase a higher-fidelity / higher-processivity polymerase.  What sort of read lengths could I typically get from a commercial sanger sequencing provider?  Like, 700-1000 bp?  I would be interested in amplifying like 5kb, if possible.

Cheers,

Mac

On Wed, Apr 14, 2010 at 7:23 PM, Bryan Bishop <kanzure@gmail.com> wrote:

---------- Forwarded message ----------
From: TS Nowlan <apokruphos@gmail.com>
Date: Wed, Apr 14, 2010 at 6:14 PM
Subject: Sequencer update
To: socal-diybio@googlegroups.com


Hey folks,

Things are moving slowly forward with our lovely sequencer, but I
thought it good to put all the docs and whatnot we've managed to find
together in one place. I've found a protocol for using it, a Windows
installer for ABI sequence analysis software (good to convert it into
a non-proprietary format for us to work with) and user's manuals for
the diagnostics system and the sequencer overall. I added links to all
that to the Lab Protocol wiki page, though I imagine it should have
its own page at some point. I'll likely be adding more this afternoon
as I find it.

Here's the link again for those that may have forgotten, or not made
accounts yet:
http://omegahelm.com/diybio/tiki-index.php?page=Lab+Protocols

And of course feel free to edit and add more stuff as you like.

-Tor

--
"And if ye cannot be saints of knowledge, then be at least its warriors."
-- Friedrich Nietzsche



--
- Bryan
http://heybryan.org/
1 512 203 0507

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-- Friedrich Nietzsche

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