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Tuesday, January 4, 2011
Monday, January 3, 2011
Sunday, January 2, 2011
Broken Cyborg (was Re: "Transcending the Human, DIY Style")
We're in it for human enhancement, synthetic biologies, longevity, nootropics, software, prosthetics,
As you can see from the x-rays (most clearly in the fourth picture), I have a titanium full knee replacement with a broken rod that takes the place of a tibia.
http://picasaweb.google.com/technologiclee/ProstheticImplantXRays#
History:
This started as an ostogenic sarcoma in the tibia at age 15.
http://www.google.com/search?q=ostogenic+sarcoma
There was a year of chemotherapy.
The knee was replaced with a cadaver bone that was shattered a year later.
The knee was then replaced by the hardware shown in the x-rays.
I had good mobility for about 10 years. Then one day the rod just broke in mid step.
This is probably due to metal fatigue.
The rod has been broken for over two years now.
My options:
I have not found a doctor that is interested in attempting to replace this hardware yet. Maybe my new health care will bring help this year.
At the time of the surgery this was a fairly new procedure. Since then it has become more commonplace, but it is still a fairly complex surgery. There would be no guarantee of improved mobility and the possibility of infection, amputation and or death.
Honestly, I do not think that the procedures currently available are what I want.
I would like something more 'natural', like having a replacement knee printed by a bone printer and installed. There is already nerve, muscle and skin damage, so for an ideal solution, stem cells would be used to regenerate the damaged tissues.
http://www.google.com/search?q=bone+printer
What would be better is to augment the knee with robotics internally or as an external brace, like they are working on at MIT Leg Lab.
http://www.ai.mit.edu/projects/leglab/
Best Solution:
Now that the future is here, the hardware and software for 'medical nanobots' is almost ready. What would this entail? Removing the hardware. Directing stem cells to sites of damage and allow them to organize into appropriate tissues. This means that a 3-D model of the leg would be made and a set of instructions prepared to direct the robots. This is the next 'Killer App'. I would like for this to be the start of a thread about medical hardware and software in terms of radical reconstruction. What is the best medical and machine control software available to base this off of? For a start there is EMC machine control software and Google Body.
http://www.google.com/search?q=medical+nanobot
http://www.google.com/search?q=EMC+machine+control
http://www.google.com/search?q=google+body
Do you remember the 'tissue processing' scene from the movie The Fifth Element? Watch the hardware and software in this clip. This is the goal. This is something that is coming together from every corner of research.
http://en.vidivodo.com/183936/the-fifth-element-part-1
Trans-dermal Implant:
During the chemotherapy I had a "port (or portacath)" installed and later removed.
http://en.wikipedia.org/wiki/Port_%28medical%29
In terms of size and considering modern electronics, that is enough volume to place something like a low power processor.
http://www.google.com/search?q=low+power+implantable+computer
http://www.google.com/search?q=implantable+computer
P.S.
Did you see the new spray on skin technique??
http://www.google.com/search?q=spray+on+skin
Saturday, January 1, 2011
Friday, December 31, 2010
Wednesday, December 29, 2010
Tuesday, December 28, 2010
Fwd: bionet.molbio.methds-reagnts - 2 new messages in 2 topics - digest
---------- Forwarded message ----------
From: "bionet.molbio.methds-reagnts group" <noreply@googlegroups.com>
Date: Dec 28, 2010 3:00 AM
Subject: bionet.molbio.methds-reagnts - 2 new messages in 2 topics - digest
To: "bionet.molbio.methds-reagnts digest subscribers" <bionet.molbio.methds-reagnts@googlegroups.com>
bionet.molbio.methds-reagnts
http://groups.google.com/group/bionet.molbio.methds-reagnts?hl=en
bionet.molbio.methds-reagnts@googlegroups.com
Today's topics:
* Methods Digest, Vol 67, Issue 8 - 1 messages, 1 author
http://groups.google.com/group/bionet.molbio.methds-reagnts/t/ca739b039a1c5a1f?hl=en
* gel elution of 6KB PCR product - 1 messages, 1 author
http://groups.google.com/group/bionet.molbio.methds-reagnts/t/fcdaf69d6c779d50?hl=en
==============================================================================
TOPIC: Methods Digest, Vol 67, Issue 8
http://groups.google.com/group/bionet.molbio.methds-reagnts/t/ca739b039a1c5a1f?hl=en
==============================================================================
== 1 of 1 ==
Date: Sun, Dec 26 2010 5:35 am
From: Virash Gupta
Dear B Ram,
try following following method. make a very small hole at the bottom
of a 0.5 ml PCR tube and cover it with some sterile glass wool. Put
your cut gel band in this tube and close it. Now put this tube into a
1.5 ml eppendorf tube. using an appropriate balancing tube spin at
12,000 rpm for 5 min. you should get 70-80% of your DNA in the
eppendorf tube which can be precipitated and resuspended in small
volume. Alternatively, put this tube into 1.5 ml tube containing ~100
microlitre TAE buffer. Also add same amount of this buffer in 0.5 ml
tube. Take two small pieces of platinum wires, dip one through space
between 0.5 ml and 1.5 ml tube so that it immerses in the buffer.
other piece of platinum wire should be purt in 0.5 ml tube buffer.
apply low current (~ 10V) with black electrode connected to wire in
0.5 ml tube and red electrode to other one immersed in 1.5 ml tube
buffer. This will make a small electrophoresis coloumn. after around
20 min, whole of DNA will move into buffer of 1.5 ml tube. Disacrd 0.5
ml tube along with its contents. precipitate DNA from buffer of 1.5 ml
tube using sod acetate and cold ethanol. All the best.
On 12/25/10, methods-request@oat.bio.indiana.edu
<methods-request@oat.bio.indiana.edu> wrote:
> Send Methods mailing list submissions to
> methods@net.bio.net
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://www.bio.net/biomail/listinfo/methods
> or, via email, send a message with subject or body 'help' to
> methods-request@net.bio.net
>
> You can reach the person managing the list at
> methods-owner@net.bio.net
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Methods digest..."
>
>
> Today's Topics:
>
> 1. gel elution of 6KB PCR product (B.Rama chandran)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sat, 25 Dec 2010 18:52:35 +0530
> From: "B.Rama chandran" <chandranbrama@gmail.com>
> Subject: gel elution of 6KB PCR product
> To: Methods@magpie.bio.indiana.edu
> Message-ID:
> <AANLkTikscKjcve5M+V_SpxhzymrfjbiQ39HRep0YQOCS@mail.gmail.com>
> Content-Type: text/plain; charset=UTF-8
>
> Dear All,
>
> I am facing problem in the gel elution of 6KB PCR product, I am
> getting very less yield (~10ng/micto liter). I am using Sigma gel
> extraction kit (catalog no:NA1111). Since yield is very less I couldn't use
> that product for restriction digestion. If you have any suggestion please
> give me. If you know any other technique which will give better yield please
> let me know that.
>
> regards,
> B.Ram.
>
>
> ------------------------------
>
> _______________________________________________
> Methods mailing list
> Methods@net.bio.net
> http://www.bio.net/biomail/listinfo/methods
>
> End of Methods Digest, Vol 67, Issue 8
> **************************************
>
--
Dr V K Gupta
Sr Microbiologist (Mol Biology)
IMBL, Department of Entomology
Pun. Agric. Univ., Ludhiana (Pb)-141004- India
M: 081465-55515
==============================================================================
TOPIC: gel elution of 6KB PCR product
http://groups.google.com/group/bionet.molbio.methds-reagnts/t/fcdaf69d6c779d50?hl=en
==============================================================================
== 1 of 1 ==
Date: Mon, Dec 27 2010 2:12 am
From: Fathi Hassan
hello,
you can try to clone the PCR product you got from gel in any cloning vector like
pJet or pGem, since they require low amounts of PCR products, then you can
digest the vector and get the fragment ready for further cloning. it is two
steps more but may help you
good luck
FH
________________________________
From: DK <dk@no.email.thankstospam.net>
To: methods@magpie.bio.indiana.edu
Sent: Sun, December 26, 2010 12:28:15 AM
Subject: Re: gel elution of 6KB PCR product
In article <mailman.445.1293295295.15153.methods@net.bio.net>, "B.Rama chandran"
<chandranbrama@gmail.com> wrote:
>Dear All,
>
> I am facing problem in the gel elution of 6KB PCR product, I am
>getting very less yield (~10ng/micto liter). I am using Sigma gel
>extraction kit (catalog no:NA1111). Since yield is very less I couldn't use
>that product for restriction digestion. If you have any suggestion please
>give me. If you know any other technique which will give better yield please
>let me know that.
What is the yield as % of input? If you didn't have much to begin
with, nothing can help. ~50% on gel-extraction are ~ normal. If it is the
yield that is poor, try the same protocol with 2 kbp. If the result is good,
your kit is not appropriate for 6K. If the result is bad, either your
reagents are gone bad/improperly prepared or you are doing something
wrong. I've purified 14 kbp from gel with about 30% using Qiagen's
kit. Never a problem.
DK
_______________________________________________
Methods mailing list
Methods@net.bio.net
http://www.bio.net/biomail/listinfo/methods
--0-1362310605-1293367365=:9174
Content-Type: text/html; charset=us-ascii
<html><head><style type="text/css"><!-- DIV {margin:0px;}
--></style></head><body><div
style="font-family:arial,helvetica,sans-serif;font-size:12pt">you can try to
clone the PCR product you got from gel in any cloning vector like pJet or pGem,
since they requier low amounts of PCR products, then you can digest the vector
and get the fragment ready for further cloning. it is two steps more but may
help you<br>good luck<br>FH<br><br><div style="font-family:
arial,helvetica,sans-serif; font-size: 12pt;"><div style="font-family:
arial,helvetica,sans-serif; font-size: 12pt;"><font face="Tahoma" size="2"><hr
size="1"><b><span style="font-weight: bold;">From:</span></b> DK
<dk@no.email.thankstospam.net><br><b><span style="font-weight:
bold;">To:</span></b> methods@magpie.bio.indiana.edu<br><b><span
style="font-weight: bold;">Sent:</span></b> Sun, December 26, 2010 12:28:15
AM<br><b><span style="font-weight: bold;">Subject:</span></b> Re: gel elution of
6KB PCR product<br></font><br>
In article <<a
ymailto="mailto:mailman.445.1293295295.15153.methods@net.bio.net"
href="mailto:mailman.445.1293295295.15153.methods@net.bio.net">mailman.445.1293295295.15153.methods@net.bio.net</a>>,
"B.Rama chandran" <<a ymailto="mailto:chandranbrama@gmail.com"
href="mailto:chandranbrama@gmail.com">chandranbrama@gmail.com</a>>
wrote:<br>>Dear All,<br>><br>> I am facing problem
in the gel elution of 6KB PCR product, I am<br>>getting very less yield
(~10ng/micto liter). I am using Sigma gel<br>>extraction kit (catalog
no:NA1111). Since yield is very less I couldn't use<br>>that product for
restriction digestion. If you have any suggestion please<br>>give me. If you
know any other technique which will give better yield please<br>>let me know
that.<br><br>What is the yield as % of input? If you didn't have much to begin
<br>with, nothing can help. ~50% on gel-extraction are ~ normal. If it
is the <br>yield that is poor, try the same protocol with 2 kbp. If the result
is good, <br>your kit is not appropriate for 6K. If the result is bad, either
your <br>reagents are gone bad/improperly prepared or you are doing something
<br>wrong. I've purified 14 kbp from gel with about 30% using Qiagen's <br>kit.
Never a problem.
<br><br>DK<br>_______________________________________________<br>Methods mailing
list<br><a ymailto="mailto:Methods@net.bio.net"
href="mailto:Methods@net.bio.net">Methods@net.bio.net</a><br><span><a
target="_blank"
href="http://www.bio.net/biomail/listinfo/methods">http://www.bio.net/biomail/listinfo/methods</a></span><br></div></div>
</div><br>
</body></html>
--0-1362310605-1293367365=:9174--
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From: "bionet.molbio.methds-reagnts group" <noreply@googlegroups.com>
Date: Dec 28, 2010 3:00 AM
Subject: bionet.molbio.methds-reagnts - 2 new messages in 2 topics - digest
To: "bionet.molbio.methds-reagnts digest subscribers" <bionet.molbio.methds-reagnts@googlegroups.com>
bionet.molbio.methds-reagnts
http://groups.google.com/group/bionet.molbio.methds-reagnts?hl=en
bionet.molbio.methds-reagnts@googlegroups.com
Today's topics:
* Methods Digest, Vol 67, Issue 8 - 1 messages, 1 author
http://groups.google.com/group/bionet.molbio.methds-reagnts/t/ca739b039a1c5a1f?hl=en
* gel elution of 6KB PCR product - 1 messages, 1 author
http://groups.google.com/group/bionet.molbio.methds-reagnts/t/fcdaf69d6c779d50?hl=en
==============================================================================
TOPIC: Methods Digest, Vol 67, Issue 8
http://groups.google.com/group/bionet.molbio.methds-reagnts/t/ca739b039a1c5a1f?hl=en
==============================================================================
== 1 of 1 ==
Date: Sun, Dec 26 2010 5:35 am
From: Virash Gupta
Dear B Ram,
try following following method. make a very small hole at the bottom
of a 0.5 ml PCR tube and cover it with some sterile glass wool. Put
your cut gel band in this tube and close it. Now put this tube into a
1.5 ml eppendorf tube. using an appropriate balancing tube spin at
12,000 rpm for 5 min. you should get 70-80% of your DNA in the
eppendorf tube which can be precipitated and resuspended in small
volume. Alternatively, put this tube into 1.5 ml tube containing ~100
microlitre TAE buffer. Also add same amount of this buffer in 0.5 ml
tube. Take two small pieces of platinum wires, dip one through space
between 0.5 ml and 1.5 ml tube so that it immerses in the buffer.
other piece of platinum wire should be purt in 0.5 ml tube buffer.
apply low current (~ 10V) with black electrode connected to wire in
0.5 ml tube and red electrode to other one immersed in 1.5 ml tube
buffer. This will make a small electrophoresis coloumn. after around
20 min, whole of DNA will move into buffer of 1.5 ml tube. Disacrd 0.5
ml tube along with its contents. precipitate DNA from buffer of 1.5 ml
tube using sod acetate and cold ethanol. All the best.
On 12/25/10, methods-request@oat.bio.indiana.edu
<methods-request@oat.bio.indiana.edu> wrote:
> Send Methods mailing list submissions to
> methods@net.bio.net
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://www.bio.net/biomail/listinfo/methods
> or, via email, send a message with subject or body 'help' to
> methods-request@net.bio.net
>
> You can reach the person managing the list at
> methods-owner@net.bio.net
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Methods digest..."
>
>
> Today's Topics:
>
> 1. gel elution of 6KB PCR product (B.Rama chandran)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sat, 25 Dec 2010 18:52:35 +0530
> From: "B.Rama chandran" <chandranbrama@gmail.com>
> Subject: gel elution of 6KB PCR product
> To: Methods@magpie.bio.indiana.edu
> Message-ID:
> <AANLkTikscKjcve5M+V_SpxhzymrfjbiQ39HRep0YQOCS@mail.gmail.com>
> Content-Type: text/plain; charset=UTF-8
>
> Dear All,
>
> I am facing problem in the gel elution of 6KB PCR product, I am
> getting very less yield (~10ng/micto liter). I am using Sigma gel
> extraction kit (catalog no:NA1111). Since yield is very less I couldn't use
> that product for restriction digestion. If you have any suggestion please
> give me. If you know any other technique which will give better yield please
> let me know that.
>
> regards,
> B.Ram.
>
>
> ------------------------------
>
> _______________________________________________
> Methods mailing list
> Methods@net.bio.net
> http://www.bio.net/biomail/listinfo/methods
>
> End of Methods Digest, Vol 67, Issue 8
> **************************************
>
--
Dr V K Gupta
Sr Microbiologist (Mol Biology)
IMBL, Department of Entomology
Pun. Agric. Univ., Ludhiana (Pb)-141004- India
M: 081465-55515
==============================================================================
TOPIC: gel elution of 6KB PCR product
http://groups.google.com/group/bionet.molbio.methds-reagnts/t/fcdaf69d6c779d50?hl=en
==============================================================================
== 1 of 1 ==
Date: Mon, Dec 27 2010 2:12 am
From: Fathi Hassan
hello,
you can try to clone the PCR product you got from gel in any cloning vector like
pJet or pGem, since they require low amounts of PCR products, then you can
digest the vector and get the fragment ready for further cloning. it is two
steps more but may help you
good luck
FH
________________________________
From: DK <dk@no.email.thankstospam.net>
To: methods@magpie.bio.indiana.edu
Sent: Sun, December 26, 2010 12:28:15 AM
Subject: Re: gel elution of 6KB PCR product
In article <mailman.445.1293295295.15153.methods@net.bio.net>, "B.Rama chandran"
<chandranbrama@gmail.com> wrote:
>Dear All,
>
> I am facing problem in the gel elution of 6KB PCR product, I am
>getting very less yield (~10ng/micto liter). I am using Sigma gel
>extraction kit (catalog no:NA1111). Since yield is very less I couldn't use
>that product for restriction digestion. If you have any suggestion please
>give me. If you know any other technique which will give better yield please
>let me know that.
What is the yield as % of input? If you didn't have much to begin
with, nothing can help. ~50% on gel-extraction are ~ normal. If it is the
yield that is poor, try the same protocol with 2 kbp. If the result is good,
your kit is not appropriate for 6K. If the result is bad, either your
reagents are gone bad/improperly prepared or you are doing something
wrong. I've purified 14 kbp from gel with about 30% using Qiagen's
kit. Never a problem.
DK
_______________________________________________
Methods mailing list
Methods@net.bio.net
http://www.bio.net/biomail/listinfo/methods
--0-1362310605-1293367365=:9174
Content-Type: text/html; charset=us-ascii
<html><head><style type="text/css"><!-- DIV {margin:0px;}
--></style></head><body><div
style="font-family:arial,helvetica,sans-serif;font-size:12pt">you can try to
clone the PCR product you got from gel in any cloning vector like pJet or pGem,
since they requier low amounts of PCR products, then you can digest the vector
and get the fragment ready for further cloning. it is two steps more but may
help you<br>good luck<br>FH<br><br><div style="font-family:
arial,helvetica,sans-serif; font-size: 12pt;"><div style="font-family:
arial,helvetica,sans-serif; font-size: 12pt;"><font face="Tahoma" size="2"><hr
size="1"><b><span style="font-weight: bold;">From:</span></b> DK
<dk@no.email.thankstospam.net><br><b><span style="font-weight:
bold;">To:</span></b> methods@magpie.bio.indiana.edu<br><b><span
style="font-weight: bold;">Sent:</span></b> Sun, December 26, 2010 12:28:15
AM<br><b><span style="font-weight: bold;">Subject:</span></b> Re: gel elution of
6KB PCR product<br></font><br>
In article <<a
ymailto="mailto:mailman.445.1293295295.15153.methods@net.bio.net"
href="mailto:mailman.445.1293295295.15153.methods@net.bio.net">mailman.445.1293295295.15153.methods@net.bio.net</a>>,
"B.Rama chandran" <<a ymailto="mailto:chandranbrama@gmail.com"
href="mailto:chandranbrama@gmail.com">chandranbrama@gmail.com</a>>
wrote:<br>>Dear All,<br>><br>> I am facing problem
in the gel elution of 6KB PCR product, I am<br>>getting very less yield
(~10ng/micto liter). I am using Sigma gel<br>>extraction kit (catalog
no:NA1111). Since yield is very less I couldn't use<br>>that product for
restriction digestion. If you have any suggestion please<br>>give me. If you
know any other technique which will give better yield please<br>>let me know
that.<br><br>What is the yield as % of input? If you didn't have much to begin
<br>with, nothing can help. ~50% on gel-extraction are ~ normal. If it
is the <br>yield that is poor, try the same protocol with 2 kbp. If the result
is good, <br>your kit is not appropriate for 6K. If the result is bad, either
your <br>reagents are gone bad/improperly prepared or you are doing something
<br>wrong. I've purified 14 kbp from gel with about 30% using Qiagen's <br>kit.
Never a problem.
<br><br>DK<br>_______________________________________________<br>Methods mailing
list<br><a ymailto="mailto:Methods@net.bio.net"
href="mailto:Methods@net.bio.net">Methods@net.bio.net</a><br><span><a
target="_blank"
href="http://www.bio.net/biomail/listinfo/methods">http://www.bio.net/biomail/listinfo/methods</a></span><br></div></div>
</div><br>
</body></html>
--0-1362310605-1293367365=:9174--
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