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Fwd: bionet.molbio.methds-reagnts - 2 new messages in 2 topics - digest
---------- Forwarded message ----------
From: "bionet.molbio.methds-reagnts group" <noreply@googlegroups.com>
Date: Dec 28, 2010 3:00 AM
Subject: bionet.molbio.methds-reagnts - 2 new messages in 2 topics - digest
To: "bionet.molbio.methds-reagnts digest subscribers" <bionet.molbio.methds-reagnts@googlegroups.com>
bionet.molbio.methds-reagnts
http://groups.google.com/group/bionet.molbio.methds-reagnts?hl=en
bionet.molbio.methds-reagnts@googlegroups.com
Today's topics:
* Methods Digest, Vol 67, Issue 8 - 1 messages, 1 author
http://groups.google.com/group/bionet.molbio.methds-reagnts/t/ca739b039a1c5a1f?hl=en
* gel elution of 6KB PCR product - 1 messages, 1 author
http://groups.google.com/group/bionet.molbio.methds-reagnts/t/fcdaf69d6c779d50?hl=en
==============================================================================
TOPIC: Methods Digest, Vol 67, Issue 8
http://groups.google.com/group/bionet.molbio.methds-reagnts/t/ca739b039a1c5a1f?hl=en
==============================================================================
== 1 of 1 ==
Date: Sun, Dec 26 2010 5:35 am
From: Virash Gupta
Dear B Ram,
try following following method. make a very small hole at the bottom
of a 0.5 ml PCR tube and cover it with some sterile glass wool. Put
your cut gel band in this tube and close it. Now put this tube into a
1.5 ml eppendorf tube. using an appropriate balancing tube spin at
12,000 rpm for 5 min. you should get 70-80% of your DNA in the
eppendorf tube which can be precipitated and resuspended in small
volume. Alternatively, put this tube into 1.5 ml tube containing ~100
microlitre TAE buffer. Also add same amount of this buffer in 0.5 ml
tube. Take two small pieces of platinum wires, dip one through space
between 0.5 ml and 1.5 ml tube so that it immerses in the buffer.
other piece of platinum wire should be purt in 0.5 ml tube buffer.
apply low current (~ 10V) with black electrode connected to wire in
0.5 ml tube and red electrode to other one immersed in 1.5 ml tube
buffer. This will make a small electrophoresis coloumn. after around
20 min, whole of DNA will move into buffer of 1.5 ml tube. Disacrd 0.5
ml tube along with its contents. precipitate DNA from buffer of 1.5 ml
tube using sod acetate and cold ethanol. All the best.
On 12/25/10, methods-request@oat.bio.indiana.edu
<methods-request@oat.bio.indiana.edu> wrote:
> Send Methods mailing list submissions to
> methods@net.bio.net
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://www.bio.net/biomail/listinfo/methods
> or, via email, send a message with subject or body 'help' to
> methods-request@net.bio.net
>
> You can reach the person managing the list at
> methods-owner@net.bio.net
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Methods digest..."
>
>
> Today's Topics:
>
> 1. gel elution of 6KB PCR product (B.Rama chandran)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sat, 25 Dec 2010 18:52:35 +0530
> From: "B.Rama chandran" <chandranbrama@gmail.com>
> Subject: gel elution of 6KB PCR product
> To: Methods@magpie.bio.indiana.edu
> Message-ID:
> <AANLkTikscKjcve5M+V_SpxhzymrfjbiQ39HRep0YQOCS@mail.gmail.com>
> Content-Type: text/plain; charset=UTF-8
>
> Dear All,
>
> I am facing problem in the gel elution of 6KB PCR product, I am
> getting very less yield (~10ng/micto liter). I am using Sigma gel
> extraction kit (catalog no:NA1111). Since yield is very less I couldn't use
> that product for restriction digestion. If you have any suggestion please
> give me. If you know any other technique which will give better yield please
> let me know that.
>
> regards,
> B.Ram.
>
>
> ------------------------------
>
> _______________________________________________
> Methods mailing list
> Methods@net.bio.net
> http://www.bio.net/biomail/listinfo/methods
>
> End of Methods Digest, Vol 67, Issue 8
> **************************************
>
--
Dr V K Gupta
Sr Microbiologist (Mol Biology)
IMBL, Department of Entomology
Pun. Agric. Univ., Ludhiana (Pb)-141004- India
M: 081465-55515
==============================================================================
TOPIC: gel elution of 6KB PCR product
http://groups.google.com/group/bionet.molbio.methds-reagnts/t/fcdaf69d6c779d50?hl=en
==============================================================================
== 1 of 1 ==
Date: Mon, Dec 27 2010 2:12 am
From: Fathi Hassan
hello,
you can try to clone the PCR product you got from gel in any cloning vector like
pJet or pGem, since they require low amounts of PCR products, then you can
digest the vector and get the fragment ready for further cloning. it is two
steps more but may help you
good luck
FH
________________________________
From: DK <dk@no.email.thankstospam.net>
To: methods@magpie.bio.indiana.edu
Sent: Sun, December 26, 2010 12:28:15 AM
Subject: Re: gel elution of 6KB PCR product
In article <mailman.445.1293295295.15153.methods@net.bio.net>, "B.Rama chandran"
<chandranbrama@gmail.com> wrote:
>Dear All,
>
> I am facing problem in the gel elution of 6KB PCR product, I am
>getting very less yield (~10ng/micto liter). I am using Sigma gel
>extraction kit (catalog no:NA1111). Since yield is very less I couldn't use
>that product for restriction digestion. If you have any suggestion please
>give me. If you know any other technique which will give better yield please
>let me know that.
What is the yield as % of input? If you didn't have much to begin
with, nothing can help. ~50% on gel-extraction are ~ normal. If it is the
yield that is poor, try the same protocol with 2 kbp. If the result is good,
your kit is not appropriate for 6K. If the result is bad, either your
reagents are gone bad/improperly prepared or you are doing something
wrong. I've purified 14 kbp from gel with about 30% using Qiagen's
kit. Never a problem.
DK
_______________________________________________
Methods mailing list
Methods@net.bio.net
http://www.bio.net/biomail/listinfo/methods
--0-1362310605-1293367365=:9174
Content-Type: text/html; charset=us-ascii
<html><head><style type="text/css"><!-- DIV {margin:0px;}
--></style></head><body><div
style="font-family:arial,helvetica,sans-serif;font-size:12pt">you can try to
clone the PCR product you got from gel in any cloning vector like pJet or pGem,
since they requier low amounts of PCR products, then you can digest the vector
and get the fragment ready for further cloning. it is two steps more but may
help you<br>good luck<br>FH<br><br><div style="font-family:
arial,helvetica,sans-serif; font-size: 12pt;"><div style="font-family:
arial,helvetica,sans-serif; font-size: 12pt;"><font face="Tahoma" size="2"><hr
size="1"><b><span style="font-weight: bold;">From:</span></b> DK
<dk@no.email.thankstospam.net><br><b><span style="font-weight:
bold;">To:</span></b> methods@magpie.bio.indiana.edu<br><b><span
style="font-weight: bold;">Sent:</span></b> Sun, December 26, 2010 12:28:15
AM<br><b><span style="font-weight: bold;">Subject:</span></b> Re: gel elution of
6KB PCR product<br></font><br>
In article <<a
ymailto="mailto:mailman.445.1293295295.15153.methods@net.bio.net"
href="mailto:mailman.445.1293295295.15153.methods@net.bio.net">mailman.445.1293295295.15153.methods@net.bio.net</a>>,
"B.Rama chandran" <<a ymailto="mailto:chandranbrama@gmail.com"
href="mailto:chandranbrama@gmail.com">chandranbrama@gmail.com</a>>
wrote:<br>>Dear All,<br>><br>> I am facing problem
in the gel elution of 6KB PCR product, I am<br>>getting very less yield
(~10ng/micto liter). I am using Sigma gel<br>>extraction kit (catalog
no:NA1111). Since yield is very less I couldn't use<br>>that product for
restriction digestion. If you have any suggestion please<br>>give me. If you
know any other technique which will give better yield please<br>>let me know
that.<br><br>What is the yield as % of input? If you didn't have much to begin
<br>with, nothing can help. ~50% on gel-extraction are ~ normal. If it
is the <br>yield that is poor, try the same protocol with 2 kbp. If the result
is good, <br>your kit is not appropriate for 6K. If the result is bad, either
your <br>reagents are gone bad/improperly prepared or you are doing something
<br>wrong. I've purified 14 kbp from gel with about 30% using Qiagen's <br>kit.
Never a problem.
<br><br>DK<br>_______________________________________________<br>Methods mailing
list<br><a ymailto="mailto:Methods@net.bio.net"
href="mailto:Methods@net.bio.net">Methods@net.bio.net</a><br><span><a
target="_blank"
href="http://www.bio.net/biomail/listinfo/methods">http://www.bio.net/biomail/listinfo/methods</a></span><br></div></div>
</div><br>
</body></html>
--0-1362310605-1293367365=:9174--
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From: "bionet.molbio.methds-reagnts group" <noreply@googlegroups.com>
Date: Dec 28, 2010 3:00 AM
Subject: bionet.molbio.methds-reagnts - 2 new messages in 2 topics - digest
To: "bionet.molbio.methds-reagnts digest subscribers" <bionet.molbio.methds-reagnts@googlegroups.com>
bionet.molbio.methds-reagnts
http://groups.google.com/group/bionet.molbio.methds-reagnts?hl=en
bionet.molbio.methds-reagnts@googlegroups.com
Today's topics:
* Methods Digest, Vol 67, Issue 8 - 1 messages, 1 author
http://groups.google.com/group/bionet.molbio.methds-reagnts/t/ca739b039a1c5a1f?hl=en
* gel elution of 6KB PCR product - 1 messages, 1 author
http://groups.google.com/group/bionet.molbio.methds-reagnts/t/fcdaf69d6c779d50?hl=en
==============================================================================
TOPIC: Methods Digest, Vol 67, Issue 8
http://groups.google.com/group/bionet.molbio.methds-reagnts/t/ca739b039a1c5a1f?hl=en
==============================================================================
== 1 of 1 ==
Date: Sun, Dec 26 2010 5:35 am
From: Virash Gupta
Dear B Ram,
try following following method. make a very small hole at the bottom
of a 0.5 ml PCR tube and cover it with some sterile glass wool. Put
your cut gel band in this tube and close it. Now put this tube into a
1.5 ml eppendorf tube. using an appropriate balancing tube spin at
12,000 rpm for 5 min. you should get 70-80% of your DNA in the
eppendorf tube which can be precipitated and resuspended in small
volume. Alternatively, put this tube into 1.5 ml tube containing ~100
microlitre TAE buffer. Also add same amount of this buffer in 0.5 ml
tube. Take two small pieces of platinum wires, dip one through space
between 0.5 ml and 1.5 ml tube so that it immerses in the buffer.
other piece of platinum wire should be purt in 0.5 ml tube buffer.
apply low current (~ 10V) with black electrode connected to wire in
0.5 ml tube and red electrode to other one immersed in 1.5 ml tube
buffer. This will make a small electrophoresis coloumn. after around
20 min, whole of DNA will move into buffer of 1.5 ml tube. Disacrd 0.5
ml tube along with its contents. precipitate DNA from buffer of 1.5 ml
tube using sod acetate and cold ethanol. All the best.
On 12/25/10, methods-request@oat.bio.indiana.edu
<methods-request@oat.bio.indiana.edu> wrote:
> Send Methods mailing list submissions to
> methods@net.bio.net
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://www.bio.net/biomail/listinfo/methods
> or, via email, send a message with subject or body 'help' to
> methods-request@net.bio.net
>
> You can reach the person managing the list at
> methods-owner@net.bio.net
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Methods digest..."
>
>
> Today's Topics:
>
> 1. gel elution of 6KB PCR product (B.Rama chandran)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sat, 25 Dec 2010 18:52:35 +0530
> From: "B.Rama chandran" <chandranbrama@gmail.com>
> Subject: gel elution of 6KB PCR product
> To: Methods@magpie.bio.indiana.edu
> Message-ID:
> <AANLkTikscKjcve5M+V_SpxhzymrfjbiQ39HRep0YQOCS@mail.gmail.com>
> Content-Type: text/plain; charset=UTF-8
>
> Dear All,
>
> I am facing problem in the gel elution of 6KB PCR product, I am
> getting very less yield (~10ng/micto liter). I am using Sigma gel
> extraction kit (catalog no:NA1111). Since yield is very less I couldn't use
> that product for restriction digestion. If you have any suggestion please
> give me. If you know any other technique which will give better yield please
> let me know that.
>
> regards,
> B.Ram.
>
>
> ------------------------------
>
> _______________________________________________
> Methods mailing list
> Methods@net.bio.net
> http://www.bio.net/biomail/listinfo/methods
>
> End of Methods Digest, Vol 67, Issue 8
> **************************************
>
--
Dr V K Gupta
Sr Microbiologist (Mol Biology)
IMBL, Department of Entomology
Pun. Agric. Univ., Ludhiana (Pb)-141004- India
M: 081465-55515
==============================================================================
TOPIC: gel elution of 6KB PCR product
http://groups.google.com/group/bionet.molbio.methds-reagnts/t/fcdaf69d6c779d50?hl=en
==============================================================================
== 1 of 1 ==
Date: Mon, Dec 27 2010 2:12 am
From: Fathi Hassan
hello,
you can try to clone the PCR product you got from gel in any cloning vector like
pJet or pGem, since they require low amounts of PCR products, then you can
digest the vector and get the fragment ready for further cloning. it is two
steps more but may help you
good luck
FH
________________________________
From: DK <dk@no.email.thankstospam.net>
To: methods@magpie.bio.indiana.edu
Sent: Sun, December 26, 2010 12:28:15 AM
Subject: Re: gel elution of 6KB PCR product
In article <mailman.445.1293295295.15153.methods@net.bio.net>, "B.Rama chandran"
<chandranbrama@gmail.com> wrote:
>Dear All,
>
> I am facing problem in the gel elution of 6KB PCR product, I am
>getting very less yield (~10ng/micto liter). I am using Sigma gel
>extraction kit (catalog no:NA1111). Since yield is very less I couldn't use
>that product for restriction digestion. If you have any suggestion please
>give me. If you know any other technique which will give better yield please
>let me know that.
What is the yield as % of input? If you didn't have much to begin
with, nothing can help. ~50% on gel-extraction are ~ normal. If it is the
yield that is poor, try the same protocol with 2 kbp. If the result is good,
your kit is not appropriate for 6K. If the result is bad, either your
reagents are gone bad/improperly prepared or you are doing something
wrong. I've purified 14 kbp from gel with about 30% using Qiagen's
kit. Never a problem.
DK
_______________________________________________
Methods mailing list
Methods@net.bio.net
http://www.bio.net/biomail/listinfo/methods
--0-1362310605-1293367365=:9174
Content-Type: text/html; charset=us-ascii
<html><head><style type="text/css"><!-- DIV {margin:0px;}
--></style></head><body><div
style="font-family:arial,helvetica,sans-serif;font-size:12pt">you can try to
clone the PCR product you got from gel in any cloning vector like pJet or pGem,
since they requier low amounts of PCR products, then you can digest the vector
and get the fragment ready for further cloning. it is two steps more but may
help you<br>good luck<br>FH<br><br><div style="font-family:
arial,helvetica,sans-serif; font-size: 12pt;"><div style="font-family:
arial,helvetica,sans-serif; font-size: 12pt;"><font face="Tahoma" size="2"><hr
size="1"><b><span style="font-weight: bold;">From:</span></b> DK
<dk@no.email.thankstospam.net><br><b><span style="font-weight:
bold;">To:</span></b> methods@magpie.bio.indiana.edu<br><b><span
style="font-weight: bold;">Sent:</span></b> Sun, December 26, 2010 12:28:15
AM<br><b><span style="font-weight: bold;">Subject:</span></b> Re: gel elution of
6KB PCR product<br></font><br>
In article <<a
ymailto="mailto:mailman.445.1293295295.15153.methods@net.bio.net"
href="mailto:mailman.445.1293295295.15153.methods@net.bio.net">mailman.445.1293295295.15153.methods@net.bio.net</a>>,
"B.Rama chandran" <<a ymailto="mailto:chandranbrama@gmail.com"
href="mailto:chandranbrama@gmail.com">chandranbrama@gmail.com</a>>
wrote:<br>>Dear All,<br>><br>> I am facing problem
in the gel elution of 6KB PCR product, I am<br>>getting very less yield
(~10ng/micto liter). I am using Sigma gel<br>>extraction kit (catalog
no:NA1111). Since yield is very less I couldn't use<br>>that product for
restriction digestion. If you have any suggestion please<br>>give me. If you
know any other technique which will give better yield please<br>>let me know
that.<br><br>What is the yield as % of input? If you didn't have much to begin
<br>with, nothing can help. ~50% on gel-extraction are ~ normal. If it
is the <br>yield that is poor, try the same protocol with 2 kbp. If the result
is good, <br>your kit is not appropriate for 6K. If the result is bad, either
your <br>reagents are gone bad/improperly prepared or you are doing something
<br>wrong. I've purified 14 kbp from gel with about 30% using Qiagen's <br>kit.
Never a problem.
<br><br>DK<br>_______________________________________________<br>Methods mailing
list<br><a ymailto="mailto:Methods@net.bio.net"
href="mailto:Methods@net.bio.net">Methods@net.bio.net</a><br><span><a
target="_blank"
href="http://www.bio.net/biomail/listinfo/methods">http://www.bio.net/biomail/listinfo/methods</a></span><br></div></div>
</div><br>
</body></html>
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Monday, December 27, 2010
sonotweezers
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Fwd: The 10 insights of the decade, 10 breakthroughs of 2010 (K21st - Essential 21st Century Knowledge)
---------- Forwarded message ----------
From: "Newsfeed to Email Gateway" <emlynoregan@gmail.com>
Date: Dec 17, 2010 11:46 AM
Subject: The 10 insights of the decade, 10 breakthroughs of 2010 (K21st - Essential 21st Century Knowledge)
To: <technologiclee@gmail.com>
From: "Newsfeed to Email Gateway" <emlynoregan@gmail.com>
Date: Dec 17, 2010 11:46 AM
Subject: The 10 insights of the decade, 10 breakthroughs of 2010 (K21st - Essential 21st Century Knowledge)
To: <technologiclee@gmail.com>
The 10 insights of the decade, 10 breakthroughs of 2010 (12/17/10 14:50:45 UTC)
From quantum mechanics, unveiling and sequencing the Neandertal genome, to Exoplanets and metamaterials, what more could we ask for?
Amplify'd from www.kurzweilai.net
"This year's Breakthrough of the Year represents the first time that scientists have demonstrated quantum effects in the motion of a human-made object," said Adrian Cho, a news writer for Science. "On a conceptual level that's cool because it extends quantum mechanics into a whole new realm. On a practical level, it opens up a variety of possibilities ranging from new experiments that meld quantum control over light, electrical currents and motion to, perhaps someday, tests of the bounds of quantum mechanics and our sense of reality."
Science's list of the nine other groundbreaking achievements from 2010 follows.
Synthetic Biology: In a defining moment for biology and biotechnology, researchers built a synthetic genome and used it to transform the identity of a bacterium. The genome replaced the bacterium's DNA so that it produced a new set of proteins—an achievement that prompted a Congressional hearing on synthetic biology. In the future, researchers envision synthetic genomes that are custom-built to generate biofuels, pharmaceuticals or other useful chemicals.
Neandertal Genome: Researchers sequenced the Neandertal genome from the bones of three female Neandertals who lived in Croatia sometime between 38,000 and 44,000 years ago. New methods of sequencing degraded fragments of DNA allowed scientists to make the first direct comparisons between the modern human genome and that of our Neandertal ancestors.
HIV Prophylaxis: Two HIV prevention trials of different, novel strategies reported unequivocal success: A vaginal gel that contains the anti-HIV drug tenofovir reduced HIV infections in women by 39 percent and an oral pre-exposure prophylaxis led to 43.8 fewer HIV infections in a group of men and transgender women who have sex with men.
Exome Sequencing/Rare Disease Genes: By sequencing just the exons of a genome, or the tiny portion that actually codes for proteins, researchers who study rare inherited diseases caused by a single, flawed gene were able to identify specific mutations underlying at least a dozen diseases.
Molecular Dynamics Simulations:Simulating the gyrations that proteins make as they fold has been a combinatorial nightmare. Now, researchers have harnessed the power of one of the world's most powerful computers to track the motions of atoms in a small, folding protein for a length of time 100 times longer than any previous efforts.
Quantum Simulator: To describe what they see in the lab, physicists cook up theories based on equations. Those equations can be fiendishly hard to solve. This year, though, researchers found a short-cut by making quantum simulators—artificial crystals in which spots of laser light play the role of ions and atoms trapped in the light stand in for electrons. The devices provide quick answers to theoretical problems in condensed matter physics and they might eventually help solve mysteries such as superconductivity.
Next-Generation Genomics: Faster and cheaper sequencing technologies are enabling very large-scale studies of both ancient and modern DNA. The 1,000 Genomes Project, for example, has already identified much of the genome variation that makes us uniquely human—and other projects in the works are set to reveal much more of the genome's function.
RNA Reprogramming:Reprogramming cells—turning back their developmental clocks to make them behave like unspecialized "stem cells" in an embryo—has become a standard lab technique for studying diseases and development. This year, researchers found a way to do it using synthetic RNA. Compared with previous methods, the new technique is twice as fast, 100 times as efficient and potentially safer for therapeutic use.
The Return of the Rat: Mice rule the world of laboratory animals, but for many purposes researchers would rather use rats. Rats are easier to work with and anatomically more similar to human beings; their big drawback is that methods used to make "knockout mice"—animals tailored for research by having specific genes precisely disabled—don't work for rats. A flurry of research this year, however, promises to bring "knockout rats" to labs in a big way.
Finally, to celebrate the end of the current decade, Science news reporters and editors have taken a step back from their weekly reporting to take a broader look at 10 of the scientific insights that have changed the face of science since the dawn of the new millennium. A list of these 10 "Insights of the Decade" follows.
The Dark Genome: Genes used to get all the glory. Now, however, researchers recognize that these protein-coding regions of the genome account for just 1.5 percent of the whole. The rest of the genome, including small coding and non-coding RNAs—previously written off as "junk"—is proving to be just as important as the genes.
Precision Cosmology: Over the past decade, researchers have deduced a very precise recipe for the content of the universe, which consists of ordinary matter, dark matter and dark energy; as well as instructions for putting it all together. These advances have transformed cosmology into a precision science with a standard theory that now leaves very little wiggle room for other ideas.
Ancient Biomolecules: The realization that "biomolecules" like ancient DNA and collagen can survive for tens of thousands of years and provide important information about long-dead plants, animals and humans has provided a boon for paleontology. Analysis of these tiny time machines can now reveal anatomical adaptations that skeletal evidence simply can't provide, such as the color of a dinosaur's feathers or how woolly mammoths withstood the cold.
Water on Mars: Half a dozen missions to Mars over the past decade have provided clear evidence that the Red Planet once harbored enough water—either on it or just inside it—to alter rock formations and, possibly, sustain life. This Martian water was probably present around the time that life was beginning to appear on Earth, but there is still enough moisture on Mars today to encourage scientists seeking living, breathing microbes.
Reprogramming Cells: During the past decade, the notion that development is a one-way street has been turned on its head. Now, researchers have figured out how to "reprogram" fully developed cells into so-called pluripotent cells that regain their potential to become any type of cell in the body. This technique has already been used to make cell lines from patients with rare diseases, but ultimately, scientists hope to grow genetically matched replacement cells, tissues and organs.
The Microbiome: A major shift in the way we view the microbes and viruses that call the human body home has led researchers to the concept of the microbiome—or the collective genomes of the host and the other creatures that live on or inside it. Since 90 percent of the cells in our bodies are actually microbial, scientists are beginning to understand how significantly microbial genes can affect how much energy we absorb from our foods and how our immune systems respond to infections.
Exoplanets: In the year 2000, researchers were aware of just 26 planets outside our solar system. By 2010, that number had jumped to 502—and still counting. With emerging technologies, astronomers expect to find abundant Earth-like planets in the universe. But for now, the sizes and orbits of larger planets already discovered are revolutionizing scientists' understanding of how planetary systems form and evolve.
Inflammation: Not long ago, inflammation was known as the simple sidekick to our healing machinery, briefly setting in to help immune cells rebuild tissue damage caused by trauma or infection. Today, however, researchers believe that inflammation is also a driving force behind the chronic diseases that will eventually kill nearly all of us, including cancer, Alzheimer's disease, atherosclerosis, diabetes and obesity.
Metamaterials: By synthesizing materials with unconventional and tunable optical properties, physicists and engineers have pioneered new ways to guide and manipulate light, creating lenses that defy the fundamental limits on resolution. They've even begun constructing "cloaks" that can make an object invisible.
Climate Change: Over the past decade, researchers have solidified some fundamental facts surrounding global climate change: The world is warming, humans are behind the warming and the natural processes of the Earth are not likely to slow that warming. But, the next 10 years will determine how scientists and policymakers proceed with this vital information.
See this Amp at http://amplify.com/u/inkq
Thursday, December 16, 2010
microscope moves atoms
A microscope that can move atoms and draw super high resolution surface images of living cells
Published with Blogger-droid v1.6.5
PCR
BioTechniques - Rapid DNA amplification in a capillary tube by natural convection with a single isothermal heater
Published with Blogger-droid v1.6.5
shroom @ home
'Shroom' in Your Kitchen With the Easy-to-Grow Mushroom Garden | Inhabitat - Green Design Will Save the World
Published with Blogger-droid v1.6.5
Wednesday, December 15, 2010
python miro community
Python Miro Community - All Python Video, All the Time
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dual core phone
LG officially announces Tegra 2 dual-core Optimus 2X | Android Central
Published with Blogger-droid v1.6.5
micro SMS analyzer
MEMS bring micro DNA analyser closer - 12/15/2010 - Electronics Weekly
Published with Blogger-droid v1.6.5
stirling energy
Stirling Energy to Kick Off Its First Plant: Cleantech News «
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desert solar breeder
Sahara Solar Breeder Project Will Turn Desert Into Energy Source | Inhabitat - Green Design Will Save the World
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floating solar panels
Solaris Synergy Unveils Floating Photovoltaic Panels | Inhabitat - Green Design Will Save the World
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wasps are solar powered
Wasps Inspire Organic Solar Cells | Inhabitat - Green Design Will Save the World
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200 miles in solar powered chair
Designer Makes 200 Miles Trek In Solar-Powered Wheelchair | Inhabitat - Green Design Will Save the World
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Python CAD
PythonCAD | Download PythonCAD software for free at SourceForge.net
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Tuesday, December 14, 2010
E3101 Series Optical Tweezer SystemsBRFONT SIZE=1ULLISelf-contained/LILISingle Beam/LILIQPD Force Measurement/LI/UL/FONT from Elliot Scientific
E3101 Series Optical Tweezer SystemsBRFONT SIZE=1ULLISelf-contained/LILISingle Beam/LILIQPD Force Measurement/LI/UL/FONT from Elliot Scientific
E3101 Series Optical Tweezer Systems
|
The E3101 Optical Tweezer System is an augmented version of our E3100, offering a simple, pre-aligned and fully interlocked, laser workstation for single beam trapping and manipulation of micron sized particles. It incorporates all the key items of hardware within a stylish self contained desktop instrument. The standard version has been designed as an economical entry-level product to enable non-specialist users to evaluate the technique in a safe class 1 laser environment. For more advanced applications, a quadrant photo detector (QPD) system can be incorporated to offer force measurement. Output from the internal colour CCD camera can be displayed either directly on a monitor via the S-video connection or on a computer fitted with a framegrabber. Features
Cell manipulation is carried out by using either the conjugate beam steering optics or the precision XY stage. Trapping in X,Y and Z axes is also possible using the variable focus stage, where particles can be lifted above the rest of the sample. For applications involving rotation of birefringent particles an optional polarization optic and rotation mount can be added. Samples on a microscope slide are inserted into the sample holder (see right) which is mounted on an XYZ stage. Access to the sample holder is via an interlocked door. The diode laser is mounted within the enclosure and in the standard system comprises either a 685nm (40 mW) or 785nm (56 mW) source. Each laser features a circular near Gaussian profile with diffraction-limited performance. The x100 (NA 1.25) oil immersion objective in conjunction with the high beam quality of the laser provides a very small focused spot allowing the trapping of individual particles. Specifications Model E3101 Optical Tweezer System comprises:
The E3101 is manufactured under license from The University of St. Andrews. |
exoskeleton leg
Can Tibion’s Bionic Leg Rewire Stroke Victims’ Brains? | Xconomy
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Monday, December 13, 2010
News from Kojo: NetBeans Platform Scala Learning Environment
News from Kojo: NetBeans Platform Scala Learning Environment | NetBeans Zone
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chrome to phone
AndroidApp - chrometophone - Project Hosting on Google Code
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fox to phone
FoxToPhone (a.k.a. SendToPhone) :: Add-ons for Firefox
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Agent procedural programming language
Agena download - Agena 1.0.5
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Sunday, December 12, 2010
video analysis software
Tracker Video Analysis and Modeling Tool for Physics Education
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Friday, December 10, 2010
Cli companion. Learn and enjoy the command line.
CLI Companion - Incredible Tool to Learn Command Line | Tech Drive-in
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Sprint Samsung Epic 4G benchmarks
Samsung Epic 4G (Sprint) - Benchmark Test Results
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meta tag for Google
Verification: Meta tag - Webmaster Tools Help
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Thursday, December 9, 2010
employment detection
Breakthrough towards lab-on-chip system for fast detection of single nucleotide variations in DNA
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Wednesday, December 8, 2010
Monday, December 6, 2010
android 2.3 changes
Android 2.3 Platform | Android Developers
"
The platform now includes a SIP protocol stack and framework API that lets developers build internet telephony applications. Using the API, applications can offer voice calling features without having to manage sessions, transport-level communication, or audio — these are handled transparently by the platform's SIP API and services.
Android 2.3 includes an NFC stack and framework API that lets developers read NDEF tags that are discovered as a user touches an NFC-enabled device to tag elements embedded in stickers, smart posters, and even other devices.
Android 2.3 adds platform and API support for several new sensor reading types — gyroscope, rotation vector, linear acceleration, gravity, and barometer.
Applications can now make use of any cameras that are available on a device, for either photo or video capture. The Camera lets applications query for the number of cameras available and the unique characteristics of each.
New parameters for cameras, including focus distance, focus mode, and preview fps maximum/minimum
The platform's media framework adds support for new per-track or global audio effects, including bass boost, headphone virtualization, equalization, and reverb.
The application uses advanced multipoint multitouch capabilities on the device screen, for tracking up to five points fully independently.
WiFi scanner graph
<?xml version='1.0' encoding='UTF-8' standalone='yes' ?><wifiScanResults generator="com.farproc.wifi.analyzer (2.5.3)" generatorUrl="market://search?q=pname:com.farproc.wifi.analyzer" number="1"><scanResult SSID="RR_BECK" BSSID="00:24:8c:79:71:a5" capabilities="[WEP]" frequency="2462" level="-74" /></wifiScanResults>
Fwd: Rev
---------- Forwarded message ----------
From: Lee Nelson <technologiclee@gmail.com>
Date: Mon, Jun 14, 2010 at 1:02 PM
Subject: Rev
To: Lee Nelson <technologiclee@gmail.com>
From: Lee Nelson <technologiclee@gmail.com>
Date: Mon, Jun 14, 2010 at 1:02 PM
Subject: Rev
To: Lee Nelson <technologiclee@gmail.com>
Title:ct cs
Sunday, December 5, 2010
Nanoengineers wiki
Welcome to your new Wikidot site - Nanoengineers
Would you like to contribute to the Nanoengineers wiki?
Saturday, December 4, 2010
Fwd: Google Alert - "xray laser"
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Date: Sat, Dec 4, 2010 at 6:12 AM
Subject: Google Alert - "xray laser"
To: technologiclee@gmail.com
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From: Google Alerts <googlealerts-noreply@google.com>
Date: Sat, Dec 4, 2010 at 6:12 AM
Subject: Google Alert - "xray laser"
To: technologiclee@gmail.com
| ||
| GainSaturated Photoionization Based Atomic InnerShell XRay Laser ... GainSaturated Photoionization Based Atomic InnerShell XRay Laser in. Neon at 850 eV. N. Rohringer1, D. Ryan2, M. Purvis2, J. Dunn1, F. Albert1, ... www.pqeconference.com/pqe2011/abstractd/220p.pdf | ||
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Friday, December 3, 2010
OPTICAL TRAP BONDING
SYSTEM AND METHOD FOR HOLOGRAPHIC OPTICAL TRAP BONDING - Patent application
I was thinking about this years ago and wrote some notes on the net. Would it count as prior art?
Thursday, December 2, 2010
Fwd: comp.lang.python - Wing IDE
From: Wingware
Hi,
Wingware has released version 3.2.12 of Wing IDE, an integrated development
environment designed specifically for the Python programming language.
This release includes the following improvements:
* Support for Stackless version 2.7
* Correctly ignore exceptions in debugger for logged exceptions
* Fix indent conversion when file had inconsistent eol characters
* Change Mako block commenting to use ##
* Fix testing tool result display when re-running similarly named tests
* 8 other minor bug fixes
See http://wingware.com/pub/wingide/3.2.12/CHANGELOG.txt for details.
*Downloads*
Wing IDE Professional http://wingware.com/downloads/wingide/3.2
Wing IDE Personal http://wingware.com/downloads/wingide-personal/3.2
Wing IDE 101 http://wingware.com/downloads/wingide-101/3.2
*About Wing IDE*
Wing IDE is an integrated development environment designed specifically for
the Python programming language. It provides powerful editing, testing, and
debugging features that help reduce development and debugging time, cut down
on coding errors, and make it easier to understand and navigate Python code.
Wing IDE can be used to develop Python code for web, GUI, and embedded
scripting applications.
Wing IDE is available in three product levels: Wing IDE Professional is
the full-featured Python IDE, Wing IDE Personal offers a reduced feature
set at a low price, and Wing IDE 101 is a free simplified version designed
for teaching entry level programming courses with Python.
Version 3.2 of Wing IDE Professional includes the following major features:
* Professional quality code editor with vi, emacs, and other keyboard
personalities
* Code intelligence for Python: Auto-completion, call tips,
goto-definition,
error indicators, smart indent and re-wrapping, and source navigation
* Advanced multi-threaded debugger with graphical UI, command line
interaction,
conditional breakpoints, data value tool tips over code, watch tool, and
externally launched and remote debugging
* Powerful search and replace options including keyboard driven and
graphical
UIs, multi-file, wild card, and regular expression search and replace
* Version control integration for Subversion, CVS, Bazaar, git,
Mercurial, and
Perforce
* Integrated unit testing for the unittest, nose, and doctest frameworks
* Many other features including project manager, bookmarks, code snippets,
OS command integration, indentation manager, PyLint integration, and
perspectives
* Extremely configurable and may be extended with Python scripts
Please refer to the feature list at http://wingware.com/wingide/features for
a detailed listing of features by product level.
System requirements are Windows 2000 or later, OS X 10.3.9 or later for
PPC or
Intel (requires X11 Server), or a recent Linux system (either 32 or 64 bit).
Wing IDE supports Python versions 2.0.x through 3.1.x and Stackless Python.
For more information, see http://wingware.com/products
*Purchasing and Upgrading*
Wing 3.2 is a free upgrade for all Wing IDE 3.0 and 3.1 users. Version 2.x
licenses cost 1/2 the normal price to upgrade.
Upgrade a license: https://wingware.com/store/upgrade
Purchase a license: https://wingware.com/store/purchase
--
Wingware | Python IDE
The Intelligent Development Environment
for Python Programmers
www.wingware.com
==============================================================================
You received this message because you are subscribed to the Google Groups "comp.lang.python"
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Hi,
Wingware has released version 3.2.12 of Wing IDE, an integrated development
environment designed specifically for the Python programming language.
This release includes the following improvements:
* Support for Stackless version 2.7
* Correctly ignore exceptions in debugger for logged exceptions
* Fix indent conversion when file had inconsistent eol characters
* Change Mako block commenting to use ##
* Fix testing tool result display when re-running similarly named tests
* 8 other minor bug fixes
See http://wingware.com/pub/wingide/3.2.12/CHANGELOG.txt for details.
*Downloads*
Wing IDE Professional http://wingware.com/downloads/wingide/3.2
Wing IDE Personal http://wingware.com/downloads/wingide-personal/3.2
Wing IDE 101 http://wingware.com/downloads/wingide-101/3.2
*About Wing IDE*
Wing IDE is an integrated development environment designed specifically for
the Python programming language. It provides powerful editing, testing, and
debugging features that help reduce development and debugging time, cut down
on coding errors, and make it easier to understand and navigate Python code.
Wing IDE can be used to develop Python code for web, GUI, and embedded
scripting applications.
Wing IDE is available in three product levels: Wing IDE Professional is
the full-featured Python IDE, Wing IDE Personal offers a reduced feature
set at a low price, and Wing IDE 101 is a free simplified version designed
for teaching entry level programming courses with Python.
Version 3.2 of Wing IDE Professional includes the following major features:
* Professional quality code editor with vi, emacs, and other keyboard
personalities
* Code intelligence for Python: Auto-completion, call tips,
goto-definition,
error indicators, smart indent and re-wrapping, and source navigation
* Advanced multi-threaded debugger with graphical UI, command line
interaction,
conditional breakpoints, data value tool tips over code, watch tool, and
externally launched and remote debugging
* Powerful search and replace options including keyboard driven and
graphical
UIs, multi-file, wild card, and regular expression search and replace
* Version control integration for Subversion, CVS, Bazaar, git,
Mercurial, and
Perforce
* Integrated unit testing for the unittest, nose, and doctest frameworks
* Many other features including project manager, bookmarks, code snippets,
OS command integration, indentation manager, PyLint integration, and
perspectives
* Extremely configurable and may be extended with Python scripts
Please refer to the feature list at http://wingware.com/wingide/features for
a detailed listing of features by product level.
System requirements are Windows 2000 or later, OS X 10.3.9 or later for
PPC or
Intel (requires X11 Server), or a recent Linux system (either 32 or 64 bit).
Wing IDE supports Python versions 2.0.x through 3.1.x and Stackless Python.
For more information, see http://wingware.com/products
*Purchasing and Upgrading*
Wing 3.2 is a free upgrade for all Wing IDE 3.0 and 3.1 users. Version 2.x
licenses cost 1/2 the normal price to upgrade.
Upgrade a license: https://wingware.com/store/upgrade
Purchase a license: https://wingware.com/store/purchase
--
Wingware | Python IDE
The Intelligent Development Environment
for Python Programmers
www.wingware.com
==============================================================================
You received this message because you are subscribed to the Google Groups "comp.lang.python"
group.
To post to this group, visit http://groups.google.com/group/comp.lang.python?hl=en
To unsubscribe from this group, send email to comp.lang.python+unsubscribe@googlegroups.com
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